KAPA Stranded mRNA Seq Prep of Larval Oyster Samples

Larval Genomic Samples - 2024

Using Zymo Quick-DNA Miniprep Plus Kit

Using Zymo Quick-RNA Miniprep Plus Kit

Using Zymo Quick-DNA/RNA Miniprep Plus Kit

Larval Genomic Samples - 2023

Purpose: Extracting DNA and RNA from larval oyster samples from ARC1 and MV1 Vibrio challenges. Samples were chosen for practice based on the number of larvae preserved in each sample (see below).

Using Zymo Quick-DNA/RNA Miniprep Plus Kit

ROD = Roseovarius Oyster Disease

Written by Jon Puritz, Amy Zyck and Maggie Schedl

ROD = Roseovarius Oyster Disease

Goal stocking density for challenge —> 50 larvae/ml

Modified protocol formatting from Natalie Ameral’s protocol
Changes include:

• Materials required section (with # of tubes per sample and suggested tube labels)
• Purpose added